Analysis of pharmacodynamic immune responses from clinical samples often includes protein measurements from serum or plasma and flow cytometry analysis of cell populations. TruCulture® is a whole blood collection and culturing system that is easily implemented at the clinical trial site and provides access to both supernatants and cell samples. After a stationary incubation at 37oC, supernatants and cells can be separated and individually analyzed for secreted protein and cell marker investigation. This application note describes a protocol for analyzing TruCulture cell samples for surface marker expression by multi-color flow cytometry immediately after supernatant separation.
- 26 Mar 2020
- Application Notes, TruCulture