Standard Flow Cytometry Protocol for Analysis of Surface Markers for TruCulture

Analysis of pharmacodynamic immune responses from clinical samples often includes protein measurements from serum or plasma and flow cytometry analysis of cell populations. TruCulture^® is a whole blood collection and culturing system that is easily implemented at the clinical trial site and provides access to both supernatants and cell samples. After a stationary incubation at 37^oC, supernatants and cells can be separated and individually analyzed for secreted protein and cell marker investigation. This application note describes a protocol for analyzing TruCulture cell samples for surface marker expression by multi-color flow cytometry immediately after supernatant separation.