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Luminex / MAP
Simoa™
Human Sample Volume Requirements
MAP VOLUME REQUIRED
SERUM OR PLASMA OTHER FLUIDS*
DiscoveryMAP 500 µL 3 mL
HumanMAP 120 µL 650 µL
InflammationMAP 60 µL 300 µL
KidneyMAP 60 µL 110 µL
CytokineMAP A 60 µL 125 µL
CytokineMAP B 50 µL 125 µL

* Cerebrospinal fluid, urine, tissue culture supernatants,  bronchoalveolar lavage, synovial fluid, tissue extracts, tears, skin washings, etc.

MTO-icon Made To Order
Simoa™ Services
BIOMARKER LLOQ*
(SERUM AND PLASMA)
LLOQ*
(UNDILUTED SAMPLES)
VOLUME REQUIRED
Serum or plasma Other fluids**
Alpha-synuclein Simoa (A-Syn Simoa) 0.20 ng/mL 0.01 ng/mL 100 µL 150 µL
Amphiregulin (AR) MTO-icon 28 pg/mL 5.6 pg/mL 100 µL 150 µL
Beta Amyloid 40 (AB40) 28 pg/mL 5.6 pg/mL 100 µL 150 µL
Beta Amyloid 42 (AB42) 47 pg/mL 4.7 pg/mL 50 µL 150 µL
B Lymphocyte Chemoattractant (BLC, CXCL13) 0.64 pg/mL 0.064 pg/mL 50 µL 150 µL
Fibroblast Growth Factor 23 (FGF-23) 0.65 pg/mL 0.1625 pg/mL 100 µL 150 µL
Glial Fibrillary Acidic Protein (GFAP) 0.70 pg/mL 0.175 pg/mL 100 µL 150 µL
Granulocyte Colony-Stimulating Factor (G-CSF) MTO-icon 0.59 pg/mL 0.059 pg/mL 50 µL 150 µL
Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) 0.036 pg/mL 0.009 pg/mL 100 µL 150 µL
Granzyme B (GranzymeB) 0.30 pg/mL 0.075 pg/mL 100 µL 150 µL
Growth/differential factor 11 (GDF-11) 5.00 pg/mL 1.25 pg/mL 100 µL 150 µL
I-309 (C-C motif chemokine ligand I) MTO-icon 0.10 pg/mL 0.025 pg/mL 100 µL 150 µL
Interferon alpha (IFN-alpha) 0.11 pg/mL 0.055 pg/mL 150 µL 150 µL
Interferon beta (IFN-beta) 0.16 pg/mL 0.04 pg/mL 100 µL 150 µL
Interferon gamma (IFN-gamma) 0.019 pg/mL 0.0095 pg/mL 150 µL 150 µL
Interferon lambda 1 (IFN-lambda 1) MTO-icon 0.090 pg/mL 0.0225 pg/mL 100 µL 150 µL
Interleukin-1 beta (IL-1 beta) 0.031 pg/mL 0.016 pg/mL 150 µL 150 µL
Interleukin-2 (IL-2) 0.050 pg/mL 0.025 pg/mL 150 µL 150 µL
Interleukin-4 (IL-4) 0.012 pg/mL 0.006 pg/mL 150 µL 150 µL
Interleukin-5 (IL-5) 0.052 pg/mL 0.026 pg/mL 150 µL 150 µL
Interleukin-6 (IL-6) 0.091 pg/mL 0.0046 pg/mL 50 µL 150 µL
Interleukin-8 (IL-8) 0.86 pg/mL 0.0287 pg/mL 50 µL 150 µL
Interleukin-9 (IL-9) MTO-icon 0.35 pg/mL 0.0875 pg/mL 100 µL 150 µL
Interleukin-10 (IL-10) 0.037 pg/mL 0.0092 pg/mL 100 µL 150 µL
Interleukin-12 Subunit p40 (IL-12p40) 7.30 pg/mL 0.730 pg/mL 50 µL 150 µL
Interleukin-12 Subunit p70 (IL-12p70) MTO-icon 0.040 pg/mL 0.020 pg/mL 150 µL 150 µL
Interleukin-13 (IL-13) 0.099 pg/mL 0.0495 pg/mL 150 µL 150 µL
Interleukin-15 (IL-15) 0.021 pg/mL 0.00525 pg/mL 150 µL 150 µL
Interleukin-17A (IL-17A) 0.048 pg/mL 0.012 pg/mL 100 µL 150 µL
Interleukin-17C (IL-17C) 0.26 pg/mL 0.13 pg/mL 150 µL 150 µL
Interleukin-17F (IL-17F)1 0.20 pg/mL 0.1 pg/mL 150 µL 150 µL
Interleukin-19 (IL-19) 1.2 pg/mL 0.3 pg/mL 100 µL 150 µL
Interleukin-21 (IL-21) 0.0053 pg/mL 0.00235 pg/mL 150 µL 150 µL
Interleukin-22 (IL-22) 0.047 pg/mL 0.01175 pg/mL 100 µL 150 µL
Interleukin-23 (IL-23) 0.039 pg/mL 0.0195 pg/mL 150 µL 150 µL
Interleukin-31 (IL-31) 0.024 pg/mL 0.006 pg/mL 100 µL 150 µL
Interleukin-33 (IL-33) 0.19 pg/mL 0.095 pg/mL 150 µL 150 µL
Kidney Injury Molecule-1 (KIM-1) MTO-icon 3.10 pg/mL 0.155 pg/mL 50 µL 150 µL
Myeloid cell surface antigen CD33 0.11 ng/mL 0.0022 ng/mL 100 µL 150 µL
Neurofilament Light Chain (NF-L) 1.00 pg/mL 0.25 pg/mL 100 µL 150 µL
Neurofilament heavy polypeptide – phosphorylated (pNF-H) 3.8 pg/mL 0.95 pg/mL 100 µL 150 µL
Oncostatin-M (OSM) MTO-icon 0.14 pg/mL 0.07 pg/mL 150 µL 150 µL
Oncostatin-M-Specific Receptor Subunit beta (OSMR) MTO-icon 0.16 ng/mL 0.0032 ng/mL 50 µL 150 µL
Phospho-Tau181 (pTau181) 2.8 pg/mL 1.4 pg/mL 150 µL 150 µL
Phospho-Tau217 (pTau217) 0.027 pg/mL 0.00675 pg/mL 100 µL 150 µL
Programmed Cell Death Protein 1 (PD-1) MTO-icon 2.3 pg/mL 0.23 pg/mL 50 µL 150 µL
Programmed Death-Ligand 1 (PD-L1) 2.1 pg/mL 0.21 pg/mL 50 µL 150 µL
Receptor Activator of Nuclear Factor Kappa B Ligand (RANKL) 0.58 pg/mL 0.145 pg/mL 100 µL 150 µL
Tau 0.22 pg/mL 0.055 pg/mL 100 µL 150 µL
Triggering Receptor Expressed On Myeloid Cells 2 Simoa (TREM2 Simoa) 0.59 ng/mL 0.59 ng/mL 100 µL 150 µL
Tumor Necrosis Factor-alpha (TNF-α) 0.078 pg/mL 0.020 pg/mL 100 µL 150 µL
Tumor Necrosis Factor Ligand Superfamily Member 14 Total (LIGHT Total) MTO-icon 0.56 pg/mL 0.14 pg/mL 100 µL 150 µL

* Lower limit of quantitation (LLOQ) represents the lowest amount of an analyte that can be quantitatively determined with acceptable precision. LLOQ is determined by performing 2-fold serial dilutions of Standard to be tested in triplicate over three runs. The percent coefficient of variation (CV) is calculated for each of the dilution replicates, and the LLOQ is determined as the concentration at which the CV is 30%.

** Cerebrospinal fluid, urine, tissue culture supernatants,  bronchoalveolar lavage, synovial fluid, tissue extracts, tears, skin washings, etc.

1 This assay did not meet performance specifications in EDTA-Plasma and results should be used for information purposes only.

  • Filter paper blood spots
  • Other fluid samples including:
    • Tissue culture supernatant – Caution- biotin concentrations must be less than 0.65µM (
    • Urine
    • Cerebrospinal Fluid
    • Peritoneal fluid
    • Cell lysates
    • BALF (broncheoalveolar lavage fluid)
    • Tissue homogenates – Procedure
    • Synovial fluid
  • Do not send whole blood
  • Contact client services to confirm sample volume requirements

If your volumes are not sufficient, please contact RBM for other options and information.