Protocols for Processing TruCulture Samples at Clinical Sites

Pharmacodynamic analysis encompasses several techniques, including protein measurements and flow cytometry analysis. TruCulture^® is a whole blood collection and culturing system that is easily implemented at clinical trial sites and provides access to samples for both secreted protein and cell population analysis. After a 37^oC stationary incubation with or without targeted stimulants, supernatants and cells can be separated and individually analyzed. This application note describes various protocols for multi-color flow cytometry analysis of TruCulture cell samples that can be adopted at any collection site.