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Neurofilament Light Chain (NF-L)

Ultrasensitive Immunoassay


Rules-Based Medicine’s (RBM) internally developed and manufactured ultrasensitive immunoassay to human neurofilament light chain (NF-L) based on the Simoa® bead technology can accurately quantitate NF-L levels in human plasma and serum. The Simoa NF-L assay is a valuable clinical research tool for pharmacodynamic analysis of autoimmune, inflammatory, and infectious diseases.

Neurofilament Light Chain (NF-L) is a soluble cytoskeletal intermediate filament protein expressed in myelinated axons. These 68 kDa protein associates with Neurofilament medium (NfM) and Neurofilament heavy (NfH) to form neurofilaments. Damage or degeneration of neurons releases neurofilaments proportionally to the severity of disease. Increased concentrations of NF-L can be measured in the cerebrospinal fluid (CSF) and blood.

NF-L correlates to the severity of neurological disorders, including traumatic brain injuries, Parkinson’s disease, Multiple Sclerosis, ALS, Dementia, Motor Neuron Disease, Creutzfeldt-Jakob disease, Huntington’s disease, and normal pressure hydrocephalus. In HIV-associated Dementia (HAD) concentrations of NF-L in the CSF are significantly elevated. RBM’s Simoa NF-L assay can be a critical tool for clinical studies into neurological diseases.


Swiss-Prot Accession Number: P07196

Alternate names for this biomarker include:

68 kDa neurofilament protein, Neurofilament triplet L protein

Simoa™ Services
Biomarker LLOQ*
(Serum and Plasma)
(Undiluted Samples)
Volume Required
Serum or plasma Other fluids**
Neurofilament Light Chain (NF-L)  1.00 pg/mL 0.25 pg/mL 100 µL 150 µL

* Lower limit of quantitation (LLOQ) represents the lowest amount of an analyte that can be quantitatively determined with acceptable precision. LLOQ is determined by performing 2-fold serial dilutions of Standard to be tested in triplicate over three runs. The percent coefficient of variation (CV) is calculated for each of the dilution replicates, and the LLOQ is determined as the concentration at which the CV is 30%.

** Cerebrospinal fluid, urine, tissue culture supernatants,  bronchoalveolar lavage, synovial fluid, tissue extracts, tears, skin washings, etc.

All assay services are performed in our CLIA-certified laboratory.
Intended for Research Use Only.