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TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 14 (LIGHT FREE) – SIMOA

DATA QUALITY

Tumor Necrosis Factor Ligand Superfamily Member 14 (LIGHT free)

Ultrasensitive Immunoassay

SimoaMyriad RBM’s internally developed and manufactured ultrasensitive immunoassay to LIGHT free (TNFSF14) based on the Simoa® bead technology can accurately quantitate sub-pg/mL levels of the free form of LIGHT in human serum and plasma. Such sensitive detection of LIGHT free is useful for clinical studies related to several inflammatory, metabolic, regenerative, and malignant disorders.

LIGHT free, also known as TNFSF14 (Tumor necrosis factor superfamily member 14), is a member of the TNF superfamily of cytokines that activates T cells and dendritic cells. LIGHT is expressed by activated lymphocytes, natural killer cells, immature dendritic cells, monocytes, and granulocytes. LIGHT can bind with a decoy receptor (DcR3), a soluble TNFSF receptor lacking transmembrane and signaling domains. Binding to DcR3 can limit LIGHT bioavailability. Thus, detection of LIGHT free can be a critical tool for clinical studies where analysis of LIGHT that can activate signaling is important.

 

Swiss-Prot Accession Number: O43557
Alternate names for this biomarker include:
Herpes virus entry mediator ligand, HVEM-L, CD258
Simoa™ Services

Biomarker

 LLOQ*
(Serum and Plasma)		 LLOQ*
(Undiluted Samples)		 Volume Required
Serum or plasma Other fluids**
Tumor Necrosis Factor Ligand Superfamily Member 14 Total (LIGHT free)  1.5 pg/mL 0.075 pg/mL 50 µL 150 µL

* Lower limit of quantitation (LLOQ) represents the lowest amount of an analyte that can be quantitatively determined with acceptable precision. LLOQ is determined by performing 2-fold serial dilutions of Standard to be tested in triplicate over three runs. The percent coefficient of variation (CV) is calculated for each of the dilution replicates, and the LLOQ is determined as the concentration at which the CV is 30%.

** Cerebrospinal fluid, urine, tissue culture supernatants,  bronchoalveolar lavage, synovial fluid, tissue extracts, tears, skin washings, etc.

All assay services are performed in our CLIA-certified laboratory.
Intended for Research Use Only.