Rules-Based Medicine (RBM) has developed an ultrasensitive immunoassay to interleukin-8 (IL-8) based on Simoa® bead technology. Being able to accurately quantitate sub-pg/mL levels of IL-8 in human serum and plasma is a valuable clinical research tool for pharmacodynamic analysis in neutrophil pathological diseases in the areas of oncology, autoimmune, and neurology.
Interleukin-8 (IL-8, CXCL8, GCP-1, or NAP-1) is a proinflammatory chemokine produced by many cell types including those involved in innate immunity, especially monocytes and epithelial cells. IL-8 induces neutrophil chemotaxis and phagocytosis in response to infections. An ELR motif, which is responsible for the protein’s angiogenic properties is at the N-terminus of the protein. IL-8 can homodimerize or can form a heterodimer with CXCL4/PF4. The monomer and homodimer forms of IL-8 bind to and induce activation of chemokine receptors CXCR1 and CXCR2.
IL-8 induction of CXCR1 triggers neutrophil antimicrobial activation and migration to sites of infection or inflammation and has been associated with obesity. IL-8 is a proinflammatory mediator in gingivitis and psoriasis and may be a factor in colorectal cancer. The angiogenic property of IL-8 has been implicated in driving angiogenesis in several cancers including gastric cancers and head & neck cancers. IL-8 is a regulator of the central nervous system (CNS) function and development and plays a key role in gliomas. High levels of maternal IL-8 have been associated with increased risk of schizophrenia and it has been implicated in cystic fibrosis.
Swiss-Prot Accession Number: P10145
Alternate names for this biomarker include:
C-X-C motif chemokine 8, Chemokine (CXC motif) ligand 8
(Serum and Plasma)
|Serum or plasma||Other fluids**|
|Interleukin-8 (IL-8)||0.86 pg/mL||0.0287 pg/mL||50 µL||150 µL|
* Lower limit of quantitation (LLOQ) represents the lowest amount of an analyte that can be quantitatively determined with acceptable precision. LLOQ is determined by performing 2-fold serial dilutions of Standard to be tested in triplicate over three runs. The percent coefficient of variation (CV) is calculated for each of the dilution replicates, and the LLOQ is determined as the concentration at which the CV is 30%.
** Cerebrospinal fluid, urine, tissue culture supernatants, bronchoalveolar lavage, synovial fluid, tissue extracts, tears, skin washings, etc.
All assay services are performed in our CLIA-certified laboratory.
Intended for Research Use Only.