Rules-Based Medicine has developed and manufactured an ultrasensitive immunoassay to interleukin-23 (IL-23) based on Simoa® bead technology. The assay is specific to fully functionalized IL-23 and does not cross-react with the p40 or p19 subunit. Being able to accurately quantitate sub-pg/mL levels of IL-23 in human serum and plasma is a valuable clinical research tool for pharmacodynamic analysis in autoimmune, inflammatory, and oncology diseases.
IL-23 belongs to the IL-12 family and is a heterodimer of the p40 and p19 subunits. Production of IL-23 is generally limited to dendritic cells, monocytes, and macrophages, in addition to B cells and endothelial cells. Potent activators of IL-23 are ligands of pattern associated molecular patterns, such as toll-like receptors (TLR-2, 4, and 8). The receptor for IL-23 is also comprised of two different subunits, IL-12Rb1 and IL-23R and signals through JAK2/Tyk2. The functional IL-23 receptor is expressed mainly on lymphocytes, such as activated T cells (most notably T cell helper type, TH, 17), gd T cells, natural killer cells, and innate lymphoid cells. From the expression pattern of the IL-23 receptor, the action of IL-23 is mainly on directing lymphocyte function and activity. One of the most notable actions of IL-23 is in promoting and enhancing the IL-17 response. While IL-23 does not differentiate naïve T cells into TH17 cells, IL-23 is a main driver for the maintenance, survival, and expansion of TH17. The interplay between these two cytokines is now commonly referred to as the IL-23/IL-17 axis. As such, targeting IL-23 is an alternative strategy in suppressing the IL-17/TH17 immune induced pathogenesis.
Alternate names for this biomarker include:
(Serum and Plasma)
|Serum or plasma||Other fluids**|
|Interleukin-23 (IL-23)||0.039 pg/mL||0.0195 pg/mL||150 µL||150 µL|
* Lower limit of quantitation (LLOQ) represents the lowest amount of an analyte that can be quantitatively determined with acceptable precision. LLOQ is determined by performing 2-fold serial dilutions of Standard to be tested in triplicate over three runs. The percent coefficient of variation (CV) is calculated for each of the dilution replicates, and the LLOQ is determined as the concentration at which the CV is 30%.
** Cerebrospinal fluid, urine, tissue culture supernatants, bronchoalveolar lavage, synovial fluid, tissue extracts, tears, skin washings, etc.
All assay services are performed in our CLIA-certified laboratory.
Intended for Research Use Only.