Interleukin-12 Subunit p70 (IL-12p70) – Simoa

Interleukin-12 Subunit 70 (IL-12p70)

Ultrasensitive Immunoassay

Rules-Based Medicine has developed and manufactured an ultrasensitive immunoassay to interleukin-12 Subunit p70 (IL-12p70)based on Simoa® bead technology. The assay is specific for IL-12p70 and does not cross-react with IL-12p35 or IL-12p40. Being able to accurately quantitate sub-pg/mL levels of IL-12p70 in human serum and plasma is a valuable clinical research tool for pharmacodynamic analysis in oncology and autoimmune diseases.

IL-12p70 is a member of the IL-12 cytokine family and its function and activity bridges the innate and adaptive immune responses. It is a heterodimer comprised of subunits p35 and p40. The receptor for IL-12p70 is a heterodimer of IL-12Rb1 and b2 and signals using Jak2 and Tyk2. IL-12p70 is produced mainly by monocytes, macrophages, and dendritic cells, and stimulated through pattern recognition receptors such as toll-like receptors (TLR). Due to its ability to promote differentiation of T cell helper type 1 (T_H1) CD4⁺ cells, induce and accelerate IFNγ production of T and natural killer cells, and negatively regulate IL-4/T_H2 and IL-2/T-regulatory cells, IL-12p70 is considered a master regulator of the T_H1 response. Indeed, genetic deficiencies in IL-12p70 or its signaling leads to host susceptibility to diseases that require T_H1 defensive immunity, such as viral and bacterial infections and various cancers. In autoimmune (psoriasis) and inflammatory (atherosclerosis, coronary artery disease) diseases with T_H1 pathogenesis, targeting IL-12p70 is an attractive therapeutic strategy. However, since IL-12p70 is critical for the T_H1 response, targeting IL-12 for therapy has led to observed increases in risks for certain types of cancers. Monitoring circulating IL-12p70 may be necessary to balance the positive and negative effects during disease.

Swiss-Prot Accession Number: P29459 P29460

Alternate names for this biomarker include:

Interleukin 12p70, Interleukin-12, IL-12

* Lower limit of quantitation (LLOQ) represents the lowest amount of an analyte that can be quantitatively determined with acceptable precision. LLOQ is determined by performing 2-fold serial dilutions of Standard to be tested in triplicate over three runs. The percent coefficient of variation (CV) is calculated for each of the dilution replicates, and the LLOQ is determined as the concentration at which the CV is 30%.

** Cerebrospinal fluid, urine, tissue culture supernatants,  bronchoalveolar lavage, synovial fluid, tissue extracts, tears, skin washings, etc.

All assay services are performed in our CLIA-certified laboratory.
Intended for Research Use Only.