I-309 (CCL1)
Ultrasensitive Immunoassay
Rules-Based Medicine’s (RBM) internally developed and manufactured ultrasensitive immunoassay for detection of C-C motif chemokine ligand 1 (CCL1 and also known as I-309) based on the Simoa® bead technology can accurately quantitate sub-pg/mL levels of CCL1 in human plasma and serum. The Simoa CCL1 assay is a valuable clinical research tool for pharmacodynamic analysis in cancer.
CCL1 is a C-C chemokine, which are small secreted proteins involved in cell trafficking. CCL1 is secreted by activated monocytes, macrophages, T cells, vascular smooth muscle cells, and umbilical vein endothelial cells. The receptor for CCL1 is CCR8, which is expressed mostly on T regulatory cells and to a lesser extent on T helper type 2 cells, monocytes, natural killer cells, and CD8 T cells. CCL1 is a potent chemoattractant of T cells and monocytes.
The expression of CCL1 has been correlated to rheumatic heart disease, and atherosclerotic plaques have been demonstrated to express CCL1. Currently, CCL1 and its receptor CCR8 has shown to play a significant role in T regulatory cell (both CD4 and CD8) infiltration into the tumor microenvironment and suppression of anti-tumor immunity. For example, in breast cancer, CCL1 expression is elevated, associated with increased T regulatory cell infiltration, and correlated with poor survival. Recently, along with IL-11, the presence of CCL1 in bronchial alveolar lavage fluid samples may be a potential diagnostic marker for lung adenocarcinoma.
Swiss-Prot Accession Number: P22362
Alternate names for this biomarker include:
Small-inducible cytokine A1, T lymphhocyte-secreted protein I-309
| Biomarker | LLOQ* (Serum and Plasma) |
LLOQ* (Undiluted Samples) |
Volume Required | |
|---|---|---|---|---|
| Serum or plasma | Other fluids** | |||
| I-309 (C-C motif chemokine ligand I) | 0.10 pg/mL | 0.025 pg/mL | 100 µL | 150 µL |
* Lower limit of quantitation (LLOQ) represents the lowest amount of an analyte that can be quantitatively determined with acceptable precision. LLOQ is determined by performing 2-fold serial dilutions of Standard to be tested in triplicate over three runs. The percent coefficient of variation (CV) is calculated for each of the dilution replicates, and the LLOQ is determined as the concentration at which the CV is 30%.
** Cerebrospinal fluid, urine, tissue culture supernatants, bronchoalveolar lavage, synovial fluid, tissue extracts, tears, skin washings, etc.
All assay services are performed in our CLIA-certified laboratory.
Intended for Research Use Only.