Biomarker LLOQ*
(Serum and Plasma)
(Undiluted Samples)
Volume Required
Serum or plasma Other fluids**
Alpha-synuclein Simoa (A-Syn Simoa) 0.20 ng/mL 0.01 ng/mL 100 µL 150 µL
Beta Amyloid 40 (AB40) 28 pg/mL 5.6 pg/mL 100 µL 150 µL
Beta Amyloid 42 (AB42) 47 pg/mL 4.7 pg/mL 50 µL 150 µL
Glial Fibrillary Acidic Protein (GFAP) 0.70 pg/mL 0.175 pg/mL 100 µL 150 µL
Myeloid cell surface antigen CD33 (CD33) 0.11 ng/mL 0.0022 ng/mL 100 µL 150 µL
Neurofilament Light Chain (NF-L) 1.00 pg/mL 0.25 pg/mL 100 µL 150 µL
Phospho-Tau181 (pTau181) 0.28 pg/mL 1.4 pg/mL 150 µL 150 µL
Tau 0.22 pg/mL 0.055 pg/mL 100 µL 150 µL
Triggering Receptor Expressed On Myeloid Cells 2 Simoa (TREM2 Simoa) 0.59 ng/mL 0.59 ng/mL 100 µL 150 µL

* Lower limit of quantitation (LLOQ) represents the lowest amount of an analyte that can be quantitatively determined with acceptable precision. LLOQ is determined by performing 2-fold serial dilutions of Standard to be tested in triplicate over three runs. The percent coefficient of variation (CV) is calculated for each of the dilution replicates, and the LLOQ is determined as the concentration at which the CV is 30%.

** Cerebrospinal fluid, urine, tissue culture supernatants,  bronchoalveolar lavage, synovial fluid, tissue extracts, tears, skin washings, etc.